After losing their Euro 2020 opener to England, Croatia were targeting a win over the Czechs to boost their Last 16 ambitions.
However, the 1-1 draw at Hampden Park means they will now have to win against Scotland to stand any chance of progressing.
Patrik Schick opened the scoring for the Czechs after successfully converting a penalty in the 37th minute.
Croatia came out firing in the second-half and immediately hit back through Ivan Perisic.
However, their new lease of life only lasted 15 minutes as the game soon returned to a slow tempo.
There were few chances throughout the encounter as both sides looked content with the point.
You can read the full report on our sister site, Football365.
Stat of the day
From OptaJoe: Ivan Perisic has scored in each of the last four major international tournaments (2014 World Cup, EURO 2016, 2018 World Cup and EURO 2020), becoming the first Croatian to score in four separate such tournaments. Rocket.
Croatia's final game in Group D will see them go head-to-head with Scotland.
With only one point to their name, Croatia need to win if they are to stand any chance of qualifying for the knockout stages.
Czech Republic, on the other hand, will take on England in their final group game.
Jaroslav Silhavy's men are already through to the next round however, a win over the Three Lions would leave them top of the group.
Thought that may be something they're happy to avoid as winners of this group will take on the runners-up of Group F - that could be Germany, Portugal or France.