To mark the partnership, 30 tickets have been gifted to the charity for the event, to highlight the number of teenager homicides in the capital last year, which was London's worst-ever annual death toll for this age group.
MMA legend Randy Couture attended a training session with children in Tottenham to launch the partnership and also discuss the growing issue.
PFL will also be making a significant donation to GUKD to allow the charity to completely revamp its website and digital output.
To launch the partnership, Ray Sefo and Simeon Powell from the PFL joined over 50 kids and young adults in Tottenham for a training session and also a talk about the role combat sport can have in changing lives.
David Edgell, co founder of GUKD, said: "We are delighted to partner PFL in London, welcome them to our GUKD Hub, and attend the PFL launch at the Copper Box Arena. Both organisations are reenergising community spirit, and are in perfect alignment."
Gloves Up Knives Down was founded in 2019, initially using connections within the world of boxing on social media to encourage youngsters into the gym and off the streets to avoid a life that may involve knife crime, injury, and in some cases, death.
The upcoming PFL London card features some of the sport's most talented fighters and marks the first London event in the company's history.
The Women's Lightweight and Featherweight Playoffs take place in London on August 20 and will be headlined by MMA superstar Kayla Harrison, who aims to become the first person to win three PFL World Championships.
The event's co-main match-up features Manchester's Brendan Loughnane fighting in front of a home crowd against 2021 PFL Finalist Chris Wade.
Tickets for the event on August 20 are now available via Ticketmaster.
Fans in the UK can catch all the action live on Channel 4 in primetime.