The current British number three will be targeting his sixth singles title at Queen's as he continues to build form ahead of Wimbeldon - which start on June 27.
Murray is the most successful player in the event's history, lifting five singles titles from 2009 to 2016. Two of those title wins, in 2013 and 2016, were followed by victories at Wimbledon.
Murray hasn't reached the final at Queen's since that 2016 triumph, with Feliciano Lopez, Marin Cilic and Matteo Berrettini winning the event since.
Cameron Norrie, who is the British number one, will also be in action and will kick off his campaign against former Wimbledon semi-finalist Grigor Dimitrov.
Norrie reached the final last year, but fell in three sets to Berrettini.
Speaking of the Italian, the 26-year-old will be looking to defend his crown, starting with a first-round clash against Britain's Dan Evans.
Ryan Peniston, who is the British number seven, has been handed arguably the toughest first round draw as he takes on French Open runner-up Casper Ruud.
Liam Brady, on the other hand, will clash with Croatia's seventh seed Marin Cilic.
Three-time grand slam winner Stan Wawrinka will also feature at the event and will face American Frances Tiafoe. Canada's Denis Shapovalov, in contrast, will play another American, Tommy Paul.
The winner will pocket just over £340,000 and the runner-up close to £184,000 out of a total prize fund of £1.84million.