When: Tuesday June 22, 20:00 BST
How to watch: ITV
England still have work to do at Euro 2020 as they attempt to secure progression as group winners.
Czech Republic meanwhile have beaten Scotland and drawn against Croatia to put themselves in a strong position, and they will fancy their chances to get something at Wembley.
Previous three meetings
Czech Republic 2-1 England (Qualifier - October 11, 2019)
The Czechs will be heartened by the fact they won the last match between these two sides, which came in Euro 2020 qualifying.
It looked like it was going to be a routine win for England when Harry Kane gave them an early lead from the spot, but Jakub Brabec replied immediately.
Five minutes from time, Zdenek Ondrasek popped up with a winner for the hosts.
England 5-0 Czech Republic (Qualifier - March 22, 2019)
It was a very different game when Czech Republic last visited Wembley, and England will be hoping for more of the same in Euro 2020.
Raheem Sterling was the star of the show as he bagged himself a hat-trick.
The other goals came from another Harry Kane penalty and a Tomas Kalas own goal.
England 2-2 Czech Republic (Friendly - August 20, 2008)
England spent the night playing catch-up when these two sides met at Wembley in 2008.
Fabio Capello's men fell behind twice but twice hauled themselves back level to earn a draw.
Former Liverpool ace Milan Baros scored the first midway through the first half, but Wes Brown equalised on the stroke of half-time.
Marek Jankulovski had the Czech Republic back in front almost immediately after the restart though, with England needing an injury time Joe Cole goal to salvage some pride from the game.
There can be little doubt that England have not hit their pre-tournament expectations yet, although that isn't necessarily a bad thing.
The primary frustration among fans appears to be the perception that England are playing with the handbrake on, with two holding midfielders preferred to some rich attacking talent on the bench.
England's struggles to create have been highlighted by Harry Kane's lacklustre start to the tournament, although Gareth Southgate has publicly assured his captain that his spot is safe for this one.
Goalkeepers: Sam Johnstone, Jordan Pickford, Aaron Ramsdale
Defenders: Ben Chilwell, Conor Coady, Reece James, Harry Maguire, Tyrone Mings, Luke Shaw, John Stones, Kieran Trippier, Kyle Walker, Ben White
Midfielders: Jude Bellingham, Jordan Henderson, Mason Mount, Kalvin Phillips, Declan Rice
Forwards: Dominic Calvert-Lewin, Phil Foden, Jack Grealish, Harry Kane, Marcus Rashford, Bukayo Saka, Jadon Sancho, Raheem Sterling
Few people expected the Czech Republic to top this group after two matches, but they are decent enough value for it right now.
They have also provided the best goal of the tournament so far through Patrik Schick's remarkable strike from the half-way line against Scotland.
However, the Czech Republic will still go into this match looking very much outgunned, so they will need to produce a good display to resist a motivated England with a point to prove.
Czech Republic squad
Goalkeepers: Tomas Vaclik, Tomas Koubek, Ales Mandous
Defenders: Jan Boril, Jakub Brabec, Vladimir Coufal, Ondrej Celustka, Pavel Kaderabek, Tomas Kalas, Ales Mateju, David Zima
Midfielders: Antonin Barak, Vladimir Darida, Adam Hlozek, Tomas Holes, Jakub Jankto, Alex Kral, Lukas Masopust, Jakub Pesek, Tomas Soucek, Petr Sevcik, Michal Sadilek
Forwards: Michael Krmencik, Tomas Pekhart, Patrik Schick, Matej Vydra
Czech Republic wins: 1
England wins: 2
Despite his struggles so far, Harry Kane remains England's best bet for a goal, and he is 12/5 to open the scoring, and he has done that twice before against this opposition.
Kane can also be backed to bag a hat-trick at 22/1, and that looks a decent punt as he tends to respond well to criticism.
England looked susceptible to set pieces against Scotland, and that may play right into the wheelhouse of Tomas Soucek.