After reaching the semi-finals in Melbourne back in 2019, Pliskova will not take part in next year's tournament due to a hand injury she suffered during a training session.
The 29-year-old Czech enjoyed a successful 2021 campaign, reaching the quarter-finals of the US Open, as well as the final of Wimbledon, where she eventually lost out to Ashleigh Barty.
Now, having been sidelined by injury, Pliskova will miss the first Grand Slam of 2022, as well as the WTA events in Adelaide and Sydney in early January.
Her announcement follows the news that both Serena Williams and Bianca Andreescu will also be absent from the competition, with Williams also out through injury, while Andreescu has said she wants to give herself time to "reset and recover" following a frantic schedule.
Speaking of her injury, Pliskova said "Unfortunately I hurt my right hand in practice yesterday and I won't be able to play in Adelaide, Sydney, and (the) Australian Open this year.
"I am sad not being able to start my season in Australia and I will miss my Australian fans very much."
The Australian Open is due to start on January 17 and run through until January 30.