The rivals will collide six years on from their 12-round battle for the European heavyweight title at the Barclaycard Arena in Hamburg, Germany.
It was Bulgarian star Pulev (29-2, 14 KOs) who came out on top on that occasion, winning a tough split decision that saw him progress in their IBF world title eliminator and claim the vacant European crown for the second time in his career.
British favourite Chisora (32-12, 23 KOs) has since faced some of the biggest names in the heavyweight division, including WBC world title challenger Dillian Whyte twice, WBA and IBF world title challenger Carlos Takam, unified heavyweight world champion Oleksandr Usyk and most recently two slugfests with former WBO champion Joseph Parker.
Two-time world title challenger Pulev, who's only losses came against modern legend Wladimir Klitschko in 2014 and former unified king Anthony Joshua in 2020, dominated Jerry Forrest to win a unanimous decision on the Sergey Kovalev vs Tervel Pulev undercard at the Kia Forum in Inglewood, California last month.
"I'm delighted to get this fight over the line," said Matchroom Sport Chairman Eddie Hearn.
"Initially we worked on some other opponents for Derek, but this fight came out of nowhere a few days ago and I absolutely love it!
"I think the strapline, 'Total Carnage', is absolutely perfect for this fight, in terms of the build-up and in terms of the fight itself.
"Expect the unexpected but expect a thriller at The O2 on July 9. Both men have been longstanding fixtures of the World Heavyweight division and it's all or nothing at The O2 for both, as defeat could lead to the end of the road.
"Fans can expect a huge undercard and a brilliant main event for another brilliant event live on DAZN."