Eddie Hearn confirmed on Sunday that the two rivals will square off in Jeddah, Saudi Arabia, for three of the four heavyweight titles.
The showdown will be live and exclusive to DAZN in 200 territories, including the UK after Joshua snubbed Sky and signed a long-term deal with the streaming company.
DAZN have big plans for Joshua - who is now also a stakeholder - but according to the bookies, it will be Usyk who gets his hand raised once again.
Usyk is 1/2 to win compared to 7/4 for Joshua while the Ukrainian is 11/4 to win by KO/TKO or disqualification.
Joshua - who admits his best chance of winning the fight is via knockout - is 3/1 to beat Usyk inside the distance.
For a repeat points victory for Usyk, the southpaw is 5/4 while Joshua is 4/1 by decision or technical decision.
The first encounter saw a competitive opening six rounds but Usyk went on to outbox Joshua during the next six and even wobbled the Brit in the final round. If there was an extra minute then he could have won via KO.
"What a roller coaster journey, fighting for the heavyweight championship of the world for the 12th consecutive time," said Joshua.
"I won the belt, unified the division won another belt, lost the belts, became two-time unified heavyweight champion and now have my date with history set to become three-time unified heavyweight champion of the World. What an opportunity.
"Fighting championship level back to back has had its pros and cons, but I decide every day to get stronger, to learn from my experiences and grow. A happy fighter is a dangerous fighter and I am the happiest and most motivated I have been.
"Thank you to the organisers, to 258MGT, Matchroom Boxing and to all the boxing fans across the world, with a special shout out to the Ukrainian fans who have been through a really tough time."